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Determining if a trait is Mendelian

Step 7:

Possible dominant or codominant gene. This step has two separate parts.

a- Raise up an F1 mutant and breed it to another unrelated normal. The results of this will help determine which inheritance pattern it follows.

Can the offspring be classified in an on/off manner?

Yes.

No.

b- Raise up F1 mutants and breed them to each other, F1 X F1 subling. The results of this will help determine which inheritance pattern it follows.

Can the offspring be classified in an on/off manner?

Yes, two distinct phenotypes appear.

Yes, there are three distinct phenotypes.

No, various degrees of the phenotype appears.





This flowchart describs the process of test breedings needed in order to determine if a trait is inherited in a mendelian fashion. Step 1, breed to an unrelated, typical normal corn. Step2, you look for mutant offspring. If there are some mutant offspring proceed to Step 7, where you breed F1 mutant to an unrelated normal as well as F1 mutant to F1 mutant. If the results of these show that the trait is either fully present or fully absent it is a mendelian dominant if there are only two phenotypes, or codominant trait if there are three clear phenotypes. Alternately if the results are many variations with some more mutant-looking and others more normal-looking, it is not a mendelian trait. If in step 2 all of the offspring look normal, then proceed to breeding F1 X F1. If this results in fully normal offspring and fully mutant offspring, with no 'between' offspring, it is a mendelian recessive trait. Otherwise if you have hatched 20 or more with no success it is probably not a menedlian trait. If in step 2 the offspring take on appearances with many variations, some more mutant-looking and others more normal-looking, it is not a mendelian trait, but it is worth rethinking your method of identifying them to see if some aspect of them is in fact either expressed or not in any particular specimen.

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